This is the only training where trainees will perform the following
1. IVF insemination: The only training in the world where you will handle oocyte cumulus complex, prepare semen, perform IVF insemination (short co-incubation), denude the eggs to check?? for fertilization (2 PN) on day 1, and monitor cleavage of the embryos on day 2 and day 3.
2. ICSI (Intracytoplasmic sperm Injection): Denude oocytes after treating with hyalase, prepare semen and PVP dishes for sperm, egg dishes, align ICSI pipettes, inject oocytes and check for fertilization on day 1 and monitor cleavage of embryos on day 2 and 3.
3. Vitrification: Set up dishes for vitrification, perform actual vitrification of oocytes and embryos using different vitrification devices,?? warm vitrified oocytes/embryos and culture them further.
4. Observe live IVF-ICSI procedures in the IVF centre